The straight line distance between Pensacola, Florida, United States and Mobile, Alabama, United States is 53 miles or 86 kilometers. The driving distance between the two cities is 55.7 miles. This is approximately 55 minutes long drive. When you leave Pensacola, Head northwest on E Brent Ln toward Springhill Dr and then take a Slight right onto the ramp to I-10 and Merge onto I-110 N. After that, take the exit onto I-10 W that enters Alabama. After that take exit 27 for US-90/Government St/US-98 and then take a slight left at AL-16/AL-42/Bankhead Tunnel/US-31/US-90/US-98. Take a right turn at S Washington Ave and a final right turn at Dauphin St will bring you to mobile.